Example HAT User Interface
HAT has a simple graphical interface allows users to set all tracking controls and parameters. There are no mysteries to the tracking algorithm -- users can see the effects of changing parameters right away. Once set, the tracking parameters can be locked in to allow for high throughput tracking of many files. No more playing with contrast levels, or guesswork about what is happening -- HAT parameters are robust and well defined, and HAT tracks the same way every time. Not sure which setting is correct? Try tracking with all of them, and compare later -- HAT will keep the results separate and you can make a better decision.
Figure 2. Graphical interface for tracking algorithm. A single frame of data (blue is bacterial channel under phase contrast illumination, red is rhodamine-actin visualized using TRITC epifluorescence microscopy) is shown, with positions of individual tracked bacteria (white crosses) and trajectories across all frames (red dots). Tracking the entire movie of 256 frames takes approximately fifteen seconds on a Pentium III class workstation.
HAT Relational Database Support