Material Transfer Agreements

Please note that after 10 years of providing these cell lines and reagents to several thousand laboratories worldwide at the Nolan Lab expense, we have changed our procedures for providing these cells and vectors.

Academic and non-profit laboratories are not required to contact the Nolan laboratory for obtaining these materials.

Note: After July 15, 2005, any requests using the older MTA forms (which are no longer available) will be returned and you will be directed to the websites below.

Companies and industrial institutes should direct inquiries by e-mail or letter directly to:

Howard Guss

Academic and non-profit laboratories may obtain the cells and vectors below from either the ATCC or Addgene. We are no longer distributing these reagents from the lab.

The following cell lines have been deposited at the ATCC (American Type Culture Collection) Safe Deposit. The Phoenix cells are not listed in ATCC's regular catalogue. To order these cells, please send your request by email to: safedep@atcc.org. An ATCC Safe Deposit representative will contact you back with ordering instructions.

Phoenix Ampho (ATCC product# SD 3443) & Eco (ATCC product# SD 3444) cell lines - second generation retroviral producer lines (MMULV-based).
PhoenixGP cell line (ATCC product# 3514) , 293T-derived containing only gag-pol for VSV-G and other pseudotyping.

The following vectors have been deposited at Addgene. Please search their online catalogs for information on obtaining these plasmids.

pCI-VSVG - VSV-G expression plasmid.
pBMN-LacZ vector - a pBabeMN based retroviral construct with the LacZ gene.

pBMN-i-EGFP vector - a pBabeMN based retroviral construct with the EMCV IRES upstream from the humanized/enhanced GFP (EGFP).
pBMN-z-i-neo vector - a pBabeMN based retroviral construct with the EMCV IRES upstream from the Neomycin resistance gene.
Felix vectors (pCPRdEnv; pLionII; pLionIIp-YFP; pTiger and pTiger-YFP) - FIV-based (Feline Immunodeficiency) vectors for delivery to non-dividing cells.